e0771 mouse breast cancer cell line  (ATCC)

Journal: Advanced Science

Article Title: Mapping the Tissue‐of‐Origins of Mesenchymal Stromal Cells in Injury Repair

doi: 10.1002/advs.202509533

Figure Lengend Snippet: BM‐MSC‐derived myofibroblasts or CAFs were not detected in distal tissue fibrosis or tumors. A) Confocal imaging of kidney sections from normal and UUO‐induced renal fibrotic Pdgfra creER ;Sp7 dre ;R26 ZT1 mice treated with tamoxifen at 2 months old. Myofibroblasts were indicated with anti‐Col1 antibody staining. B) Confocal imaging of lung sections from normal and bleomycin‐induced pulmonary fibrotic Pdgfra creER ;Sp7 dre ;R26 ZT1 mice treated with tamoxifen at 2 months old. Myofibroblasts were indicated with anti‐Col1 antibody staining. C) Confocal imaging of liver sections from normal and CCl 4 ‐induced liver fibrotic Pdgfra creER ;Sp7 dre ;R26 ZT1 mice treated with tamoxifen at 2 months old. Myofibroblasts were indicated with anti‐Col1 antibody staining. D) Confocal imaging of liver sections from normal and DDC‐induced liver fibrotic Pdgfra creER ;Sp7 dre ;R26 ZT1 mice treated with tamoxifen at 2 months old. Myofibroblasts were indicated with anti‐Col1 antibody staining. E–H) Quantification of the percentages of DAPI + Col1 + myofibroblasts that were ZsGreen + and tdTomato + in renal fibrosis (E), pulmonary fibrosis (F), CCl 4 ‐induced liver fibrosis (G) and DDC‐induced liver fibrosis (H). n = 5 mice from 4 independent experiments. I) Confocal imaging of E0771‐induced subcutaneous tumors from Pdgfra creER ;Sp7 dre ;R26 ZT1 mice treated with tamoxifen at 2 months old. Cancer associated fibroblasts (CAFs) were indicated with anti‐Col1 antibody staining. J) Confocal imaging of normal colons and AOM/DSS‐induced colorectal cancer from Pdgfra creER ;Sp7 dre ;R26 ZT1 mice treated with tamoxifen at 2 months old. CAFs were indicated with anti‐Col1 antibody staining. K,L) Quantification of the percentages of DAPI + Col1 + CAFs that were ZsGreen + and tdTomato + in subcutaneous tumors (K) and colorectal cancer (L). n = 3 mice from 3 independent experiments.

Article Snippet: The E0771 mouse breast cancer cell line (CRL‐3461, RRID:CVCL_GR23) was obtained from the American Tissue Type Collection (ATCC) in 2018.

Techniques: Derivative Assay, Imaging, Staining

Journal: Nature Communications

Article Title: STING suppresses bone cancer pain via immune and neuronal modulation

doi: 10.1038/s41467-021-24867-2

Figure Lengend Snippet: a In vivo bioluminescence of flux emitted by LL/2-Luc2 carcinoma (LLC) cells in tumor bearing femur after vehicle or DMXAA treatment (2 × 20 mg/kg, i.p.) measured at d8, d11, and d15 post tumor inoculation ( n = 10 vehicle-treated mice, n = 11 DMXAA-treated mice). Images (left) were obtained at 15 min after i.p. injection of D-luciferin (30 mg/kg). Right, experimental scheme and quantification of a . b Ratio of maximum thigh circumference reflecting local tumor burden in mice with each indicated treatment on d17 after LLC implantation. Left, vehicle or DMXAA treatment (2 × 20 mg/kg, i.p.; n = 11 vehicle-treated mice and n = 9 DMXAA-treated mice). Right, vehicle, ADU-S100 (2 × 20 mg/kg, i.p.) or ZA (zoledronic acid; 2 × 100 µg/kg, i.p.) treatment ( n = 8 vehicle-treated mice and n = 7 ADU-S100-treated mice, and n = 8 ZA-treated mice), *** P < 0.001. c Ratio of maximum thigh circumference in mice administered with vehicle, DMXAA or ADU-S100 (2 × 100 µg/kg, i.p.) on d17 after implantation of E0771 breast cancer cells ( n = 7 mice/group), *** P < 0.001. d Images of lung tumor nodules in mice with each indicated treatment on d17 after LLC inoculation. Left, representative dorsal and ventral murine lung image, with arrows showing metastatic tumor nodules. Right, H&E staining for sections from lung samples in Left. Arrows indicates the areas with tumor cells, scale bar, 2 mm. 1 and 2 are enlarged images showing tumor tissue and peritumoral areas, respectively. Scale bar, 50 µm. Note that tumor cells have large and irregular nuclei with loss of the normal alveolar structure. e Quantification of panel d ( n = 8 vehicle-treated mice, n = 7 ADU-S100-treated mice, and n = 8 ZA-treated mice). f - g FACS analysis of CD4 + and CD8 + T cells ( f ) or Treg cells ( g ) within the bone marrow tumor microenvironment in mice treated with vehicle or DMXAA (2 × 20 mg/kg, i.p.) on d8 post-LLC inoculation ( n = 5 mice/group). The gating strategy for this figure is provided in Supplementary Fig. 7. h Local tumor burden as determined by the ratio of maximum thigh circumference after vehicle or DMXAA treatment in WT ( n = 10 mice for vehicle or DMXAA group) and Rag1 −/− mice ( n = 13 mice for vehicle or DMXAA group) on d17 after LLC implantation, *** P < 0.001. i Local tumor burden as determined by the ratio of maximum thigh circumference in Batf3 +/+ and Batf3 −/− mice with indicated treatment measured on d17 after LLC implantation ( n = 8 mice/group), *** P < 0.001. Data indicate the mean ± SEM, repeated-measures two-way ANOVA with Bonferroni’s post hoc test ( a , b , c , h , i ); one-way ANOVA with Bonferroni’s post hoc test ( e ); two-tailed Student’s t -test ( f , g ). Source data are provided as a Source Data file.

Article Snippet: The mouse E0771 breast cancer cell line (94A001) was obtained from CH3 BioSystems.

Techniques: In Vivo, Injection, Staining, Two Tailed Test